🧪 Scientific Methodology

Step	Category	Tools & Resources
1	Gene Optimization	NCBI, UniProt, KEGG, JCat, Benchling
2	Pathway Modeling	COBRApy, KBase, BioCyc
3	Enzyme Engineering	AMDock, SwissDock, AutoDock Vina, GROMACS
4	Genome Engineering	SnapGene, Benchling, CHOPCHOP, Geneious
5	Expression Validation	Galaxy, CLC Genomics, DESeq2
6	Predictive Modeling	SciPy, NumPy, scikit-learn, TensorFlow
7	Ethics & Safety	NASA Guidelines, iGEM Safety Hub

🌍 Mars Environment Challenges

• Temperature: -80°C to 20°C (avg -63°C)
• Pressure: 0.6% of Earth's atmospheric pressure
• Radiation: High UV and cosmic radiation
• Perchlorate concentration: 0.5% by weight in regolith
• Water availability: Limited, mostly ice